{"id":1033,"date":"2026-01-14T09:00:00","date_gmt":"2026-01-14T08:00:00","guid":{"rendered":"https:\/\/helexproject.eu\/?p=1033"},"modified":"2026-04-13T16:19:53","modified_gmt":"2026-04-13T14:19:53","slug":"helex-pub-3","status":"publish","type":"post","link":"https:\/\/helexproject.eu\/de\/helex-pub-3\/","title":{"rendered":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling"},"content":{"rendered":"\n<p><strong>Authors:<\/strong> Kimon Ionas, Mirjana Vukosavljev, Emilija Buli\u0107, Aleksandra Radanovi\u0107, Sini\u0161a Joci\u0107, Ankica Kondi\u0107-\u0160pika, Dragana Miladinovi\u0107<\/p>\n\n\n\n<p><strong>Journal:<\/strong> <em>International Journal of Molecular Sciences<\/em>, 27(2), January 2026<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Abstract<\/h3>\n\n\n\n<p>This study presents the first integrated analysis of genotype, medium interactions and temporal morphogenesis profiling in sunflower regeneration. It aims to characterise genotype-specific responses, identify predictive morphological markers, and develop a scalable framework for breeding and transformation. Eighteen sunflower genotypes were evaluated to assess organogenic performance, and the model genotype Ha-26-PR was used in a complementary experiment testing varying sucrose concentrations to examine their influence on morphogenic outcomes.<\/p>\n\n\n\n<p>Hierarchical Cluster Analysis, guided by the Elbow method, identified four optimal clusters aligned with three biologically meaningful categories: High Regenerators, Moderate\/Specific Regenerators, and Non-Regenerators. On S1 medium, NO-SU-12 and AS-1-PR showed superior shoot regeneration, while on R4 medium, HA-26-PR-SU and NO-SU-12 performed best. NO-SU-12 and AS-1-PR consistently excelled across both media, whereas AB-OR-8 and FE-7 remained non-regenerators. Medium R4 supported superior regeneration, primarily through root formation, while S1 failed to induce roots in any genotype, highlighting the importance of hormonal composition.<\/p>\n\n\n\n<p>Although sucrose promoted callus induction, it did not trigger organogenesis, and callus presence was weakly and unstably correlated with shoot and root formation. Instead, root formation at 14 days emerged as a robust early predictor of organogenic success. By integrating morphological, temporal and statistical analyses, this work offers a genotype-tailored regeneration framework with direct applications in molecular breeding and CRISPR\/Cas-based genome editing, contributing to HelEx\u2019s broader effort to accelerate sunflower improvement for climate resilience and pollinator-friendly traits.<\/p>\n\n\n\n<p><strong>Open Access:<\/strong> Yes &nbsp;\u2022&nbsp; <strong>Peer-reviewed:<\/strong> Yes &nbsp;\u2022&nbsp; <strong>DOI:<\/strong> <a href=\"https:\/\/doi.org\/10.3390\/ijms27020809\" target=\"_blank\" rel=\"noopener\">10.3390\/ijms27020809<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Full paper<\/h3>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/helexproject.eu\/wp-content\/uploads\/2026\/04\/ijms-27-00809-with-cover.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Einbettung von ijms-27-00809-with-cover.pdf.\"><\/object><a href=\"https:\/\/helexproject.eu\/wp-content\/uploads\/2026\/04\/ijms-27-00809-with-cover.pdf\">ijms-27-00809-with-cover.pdf<\/a><a href=\"https:\/\/helexproject.eu\/wp-content\/uploads\/2026\/04\/ijms-27-00809-with-cover.pdf\" class=\"wp-block-file__button wp-element-button\" download>Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-1033","post","type-post","status-publish","format-standard","hentry","category-publications"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project<\/title>\n<meta name=\"description\" content=\"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/helexproject.eu\/de\/helex-pub-3\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project\" \/>\n<meta property=\"og:description\" content=\"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/helexproject.eu\/de\/helex-pub-3\/\" \/>\n<meta property=\"og:site_name\" content=\"HelEx Project\" \/>\n<meta property=\"article:published_time\" content=\"2026-01-14T08:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-04-13T14:19:53+00:00\" \/>\n<meta name=\"author\" content=\"Julien\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@HelexProject\" \/>\n<meta name=\"twitter:site\" content=\"@HelexProject\" \/>\n<meta name=\"twitter:label1\" content=\"Verfasst von\" \/>\n\t<meta name=\"twitter:data1\" content=\"Julien\" \/>\n\t<meta name=\"twitter:label2\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/\"},\"author\":{\"name\":\"Julien\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#\\\/schema\\\/person\\\/63ee59bc3013eaf8e6bc06d2a291e694\"},\"headline\":\"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling\",\"datePublished\":\"2026-01-14T08:00:00+00:00\",\"dateModified\":\"2026-04-13T14:19:53+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/\"},\"wordCount\":290,\"publisher\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#organization\"},\"articleSection\":[\"Publications\"],\"inLanguage\":\"de\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/\",\"url\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/\",\"name\":\"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#website\"},\"datePublished\":\"2026-01-14T08:00:00+00:00\",\"dateModified\":\"2026-04-13T14:19:53+00:00\",\"description\":\"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/#breadcrumb\"},\"inLanguage\":\"de\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/helex-pub-3\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#website\",\"url\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/\",\"name\":\"HelEx Project\",\"description\":\"Facing climate change with a new generation of sunflowers\",\"publisher\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"de\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#organization\",\"name\":\"HelEx Project\",\"url\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"de\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/helexproject.eu\\\/wp-content\\\/uploads\\\/2023\\\/09\\\/Logo_HelEx_site.svg\",\"contentUrl\":\"https:\\\/\\\/helexproject.eu\\\/wp-content\\\/uploads\\\/2023\\\/09\\\/Logo_HelEx_site.svg\",\"width\":592,\"height\":183,\"caption\":\"HelEx Project\"},\"image\":{\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/x.com\\\/HelexProject\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/helexproject\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/#\\\/schema\\\/person\\\/63ee59bc3013eaf8e6bc06d2a291e694\",\"name\":\"Julien\",\"sameAs\":[\"https:\\\/\\\/helexproject.eu\"],\"url\":\"https:\\\/\\\/helexproject.eu\\\/de\\\/author\\\/julien\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project","description":"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/helexproject.eu\/de\/helex-pub-3\/","og_locale":"de_DE","og_type":"article","og_title":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project","og_description":"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).","og_url":"https:\/\/helexproject.eu\/de\/helex-pub-3\/","og_site_name":"HelEx Project","article_published_time":"2026-01-14T08:00:00+00:00","article_modified_time":"2026-04-13T14:19:53+00:00","author":"Julien","twitter_card":"summary_large_image","twitter_creator":"@HelexProject","twitter_site":"@HelexProject","twitter_misc":{"Verfasst von":"Julien","Gesch\u00e4tzte Lesezeit":"2\u00a0Minuten"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/#article","isPartOf":{"@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/"},"author":{"name":"Julien","@id":"https:\/\/helexproject.eu\/de\/#\/schema\/person\/63ee59bc3013eaf8e6bc06d2a291e694"},"headline":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling","datePublished":"2026-01-14T08:00:00+00:00","dateModified":"2026-04-13T14:19:53+00:00","mainEntityOfPage":{"@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/"},"wordCount":290,"publisher":{"@id":"https:\/\/helexproject.eu\/de\/#organization"},"articleSection":["Publications"],"inLanguage":"de"},{"@type":"WebPage","@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/","url":"https:\/\/helexproject.eu\/de\/helex-pub-3\/","name":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling - HelEx Project","isPartOf":{"@id":"https:\/\/helexproject.eu\/de\/#website"},"datePublished":"2026-01-14T08:00:00+00:00","dateModified":"2026-04-13T14:19:53+00:00","description":"First integrated analysis of genotype, medium interactions in sunflower regeneration (IJMS, January 2026).","breadcrumb":{"@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/#breadcrumb"},"inLanguage":"de","potentialAction":[{"@type":"ReadAction","target":["https:\/\/helexproject.eu\/de\/helex-pub-3\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/helexproject.eu\/de\/helex-pub-3\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/helexproject.eu\/de\/"},{"@type":"ListItem","position":2,"name":"Beyond the bottleneck: predicting regeneration potential in sunflower through integrated morphological and statistical profiling"}]},{"@type":"WebSite","@id":"https:\/\/helexproject.eu\/de\/#website","url":"https:\/\/helexproject.eu\/de\/","name":"HelEx Project","description":"Facing climate change with a new generation of sunflowers","publisher":{"@id":"https:\/\/helexproject.eu\/de\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/helexproject.eu\/de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"de"},{"@type":"Organization","@id":"https:\/\/helexproject.eu\/de\/#organization","name":"HelEx Project","url":"https:\/\/helexproject.eu\/de\/","logo":{"@type":"ImageObject","inLanguage":"de","@id":"https:\/\/helexproject.eu\/de\/#\/schema\/logo\/image\/","url":"https:\/\/helexproject.eu\/wp-content\/uploads\/2023\/09\/Logo_HelEx_site.svg","contentUrl":"https:\/\/helexproject.eu\/wp-content\/uploads\/2023\/09\/Logo_HelEx_site.svg","width":592,"height":183,"caption":"HelEx Project"},"image":{"@id":"https:\/\/helexproject.eu\/de\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/x.com\/HelexProject","https:\/\/www.linkedin.com\/company\/helexproject"]},{"@type":"Person","@id":"https:\/\/helexproject.eu\/de\/#\/schema\/person\/63ee59bc3013eaf8e6bc06d2a291e694","name":"Julien","sameAs":["https:\/\/helexproject.eu"],"url":"https:\/\/helexproject.eu\/de\/author\/julien\/"}]}},"_links":{"self":[{"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/posts\/1033","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/comments?post=1033"}],"version-history":[{"count":1,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/posts\/1033\/revisions"}],"predecessor-version":[{"id":1153,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/posts\/1033\/revisions\/1153"}],"wp:attachment":[{"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/media?parent=1033"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/categories?post=1033"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/helexproject.eu\/de\/wp-json\/wp\/v2\/tags?post=1033"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}